3EIG
Crystal structure of a methotrexate-resistant mutant of human dihydrofolate reductase
Summary for 3EIG
Entry DOI | 10.2210/pdb3eig/pdb |
Descriptor | Dihydrofolate reductase, METHOTREXATE, SULFATE ION, ... (5 entities in total) |
Functional Keywords | oxidoreductase, mutant human dihydrofolale reductase, nadp, one-carbon metabolism |
Biological source | Homo sapiens (Human) |
Total number of polymer chains | 1 |
Total formula weight | 22695.88 |
Authors | Yachnin, B.J.,Berghuis, A.M. (deposition date: 2008-09-15, release date: 2009-05-26, Last modification date: 2023-08-30) |
Primary citation | Volpato, J.P.,Yachnin, B.J.,Blanchet, J.,Guerrero, V.,Poulin, L.,Fossati, E.,Berghuis, A.M.,Pelletier, J.N. Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance. J.Biol.Chem., 284:20079-20089, 2009 Cited by PubMed: 19478082DOI: 10.1074/jbc.M109.018010 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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