30JS

X-ray structure of lysozyme treated with V(V)-lactate complex (structure A)

This is a non-PDB format compatible entry.

Summary for 30JS

• Entry DOI: 10.2210/pdb30js/pdb
• Descriptor: Lysozyme C, ACETATE ION, CHLORIDE ION, ... (7 entities in total)
• Functional Keywords: protein metalation, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 14970.10

Authors

Paolillo, M.,Ferraro, G.,Merlino, A. (deposition date: 2026-04-29, release date: 2026-09-30)

Primary citation

Paolillo, M.,Cuomo, V.,Ferraro, G.,Imbimbo, P.,Gumerova, N.I.,Pisanu, F.,Garribba, E.,Rompel, A.,Merlino, A.
Speciation, Protein Binding, Biotransformation, and Cytotoxicity of a VV-Lactate Complex.
Inorg.Chem., 65:19783-19798, 2026

PubMed Abstract: We studied the speciation, protein binding, biotransformation, and cytotoxicity of the dioxidovanadium(V) lactate complex Cs2[VV2O4(lact)2]·2H2O and compared the results with those obtained for the analogous malate compound. 51V NMR and ESI-MS results show that Cs2[VV2O4(lact)2]·2H2O forms [VVO2]+, [H2VVO4]-, [H2VV2O7]2-, [VV2O4(lact)2]2-, [VV3O7(lact)2]3-, [VV4O12]4-, [VV5O15]5-, [VVO2(lact)(H2O)]-, [VVO2(lact)(OH)]2-, and [VV10O28]6- species in aqueous solution. In the presence of lysozyme, the amounts of [VV10O28]6- and [VVO2(lact)(H2O)]- significantly decrease and protein adducts with [VV2O4(lact)2]2- and [VVO(lact)2]- are detected by ESI-MS. X-ray structures of the adducts show noncovalent binding of [VIVO]2+, [VVO2]+, [VV2O4(lact)2]2-, cyclic [VV3O9]3-, and [VV3O7(lact)2]3- to lysozyme. Cs2[VV2O4(lact)2]·2H2O and Cs2[VV2O4(mal)2]·2H2O exhibit higher cytotoxicity than cisplatin on PC-3 cancer cells (IC50 values are 5.9 ± 0.3 and 5.0 ± 0.3 μM, respectively), while they are less active than cisplatin against HeLa cells and less selective against BALB/c-3T3 and HaCaT cells. In systems containing [VV2O4(lact)2]2- and biological reductants, EPR studies demonstrate the formation of hydroxyl radicals, supporting a mechanism in which redox cycling between VV and VIV contributes to the oxidative stress that accounts for the observed biological activity.

PubMed: 42686708
DOI: 10.1021/acs.inorgchem.6c02377

Experimental method

X-RAY DIFFRACTION (1.2 Å)