30JS

X-ray structure of lysozyme treated with V(V)-lactate complex (structure A)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	77.850, 77.850, 37.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.048 - 1.200
Rwork	0.174
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.908
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.050	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	36597	1770
<I/σ(I)>	19.7
Completeness [%]	100.0
Redundancy	12.2
CC(1/2)	0.999	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	1.1 M sodium chloride, 0.1 M sodium acetate pH 4.0