2ZZN
The complex structure of aTrm5 and tRNACys
Summary for 2ZZN
| Entry DOI | 10.2210/pdb2zzn/pdb |
| Descriptor | Uncharacterized protein MJ0883, RNA (71-MER), S-ADENOSYLMETHIONINE, ... (5 entities in total) |
| Functional Keywords | protein-rna complex, transferase-rna complex, transferase/rna |
| Biological source | Methanocaldococcus jannaschii (Methanococcus jannaschii) More |
| Cellular location | Cytoplasm (Potential): Q58293 |
| Total number of polymer chains | 4 |
| Total formula weight | 127369.63 |
| Authors | Goto-Ito, S.,Ito, T.,Yokoyama, S. (deposition date: 2009-02-19, release date: 2009-09-15, Last modification date: 2023-11-01) |
| Primary citation | Goto-Ito, S.,Ito, T.,Kuratani, M.,Bessho, Y.,Yokoyama, S. Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation. Nat.Struct.Mol.Biol., 16:1109-1115, 2009 Cited by PubMed Abstract: tRNA precursors undergo a maturation process, involving nucleotide modifications and folding into the L-shaped tertiary structure. The N1-methylguanosine at position 37 (m1G37), 3' adjacent to the anticodon, is essential for translational fidelity and efficiency. In archaea and eukaryotes, Trm5 introduces the m1G37 modification into all tRNAs bearing G37. Here we report the crystal structures of archaeal Trm5 (aTrm5) in complex with tRNA(Leu) or tRNA(Cys). The D2-D3 domains of aTrm5 discover and modify G37, independently of the tRNA sequences. D1 is connected to D2-D3 through a flexible linker and is designed to recognize the shape of the tRNA outer corner, as a hallmark of the completed L shape formation. This interaction by D1 lowers the K(m) value for tRNA, enabling the D2-D3 catalysis. Thus, we propose that aTrm5 provides the tertiary structure checkpoint in tRNA maturation. PubMed: 19749755DOI: 10.1038/nsmb.1653 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.95 Å) |
Structure validation
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