2ZZN
The complex structure of aTrm5 and tRNACys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.838, 106.537, 134.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.690 - 2.950 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zzm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 25625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop, vapor diffusion | 7.5 | 293 | 87.5mM Mg(HCOO)2, 4.38% PEG 3350, 43.8mM CH3COONa pH 4.6, 8.8% MPD, 0.4mM CYMAL-5, 12.5mM Na-HEPES pH 7.5, sitting drop, vapor diffusion, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Mg(HCOO)2 | ||
| 10 | 1 | 2 | CH3COONa | ||
| 11 | 1 | 2 | MPD | ||
| 12 | 1 | 2 | CYMAL-5 | ||
| 13 | 1 | 2 | Na-HEPES | ||
| 14 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | CH3COONa | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | CYMAL-5 | ||
| 6 | 1 | 1 | Na-HEPES | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | Mg(HCOO)2 | ||
| 9 | 1 | 2 | PEG 3350 |
