TNF Receptor Subtype One-selective TNF Mutant with Antagonistic Activity; R1antTNF-T8

Summary for 2ZPX

Related1TNF 1TNR 2EA7 2ZJC
DescriptorTumor necrosis factor (2 entities in total)
Functional Keywordstumor necrosis factor, trimer, antagonistic activity, tnfr1 specific, phage display system, cytokine, cell membrane, lipoprotein, membrane, myristate, phosphoprotein, polymorphism, secreted, signal-anchor, transmembrane
Biological sourceHomo sapiens (Human)
Cellular locationCell membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted P01375
Total number of polymer chains3
Total molecular weight51850.31
Mukai, Y.,Nakamura, T.,Yamagata, Y.,Tsutsumi, Y. (deposition date: 2008-07-29, release date: 2009-03-24, Last modification date: 2011-07-13)
Primary citation
Mukai, Y.,Nakamura, T.,Yoshioka, Y.,Shibata, H.,Abe, Y.,Nomura, T.,Taniai, M.,Ohta, T.,Nakagawa, S.,Tsunoda, S.,Kamada, H.,Yamagata, Y.,Tsutsumi, Y.
Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF: useful information for designing artificial proteo-antagonists
J.Biochem., 146:167-172, 2009
PubMed: 19386778 (PDB entries with the same primary citation)
DOI: 10.1093/jb/mvp065
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.308366.3%5.6%4.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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