2ZNE

Crystal structure of Zn2+-bound form of des3-23ALG-2 complexed with Alix ABS peptide

2ZNE の概要

• エントリーDOI: 10.2210/pdb2zne/pdb
• 関連するPDBエントリー: 2ZN8 2ZN9 2ZND
• 分子名称: Programmed cell death protein 6, 16-meric peptide from Programmed cell death 6-interacting protein, ZINC ION, ... (5 entities in total)
• 機能のキーワード: penta-ef-hand protein, calcium binding protein, apoptosis, endoplasmic reticulum, membrane, nucleus, polymorphism, cytoplasm, host-virus interaction, protein transport, transport
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus membrane; Peripheral membrane protein: O75340; Cytoplasm, cytosol: Q8WUM4
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43867.30

構造登録者

Suzuki, H.,Kawasaki, M.,Inuzuka, T.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M. (登録日: 2008-04-22, 公開日: 2008-09-09, 最終更新日: 2023-11-01)

主引用文献

Suzuki, H.,Kawasaki, M.,Inuzuka, T.,Okumura, M.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M.
Structural Basis for Ca(2+)-Dependent Formation of ALG-2/Alix Peptide Complex: Ca(2+)/EF3-Driven Arginine Switch Mechanism
Structure, 16:1562-1573, 2008

PubMed Abstract: ALG-2 belongs to the penta-EF-hand (PEF) protein family and interacts with various intracellular proteins, such as Alix and TSG101, that are involved in endosomal sorting and HIV budding. Through X-ray crystallography, we solved the structures of Ca(2+)-free and -bound forms of N-terminally truncated human ALG-2 (des3-20ALG-2), Zn(2+)-bound form of full-length ALG-2, and the structure of the complex between des3-23ALG-2 and the peptide corresponding to Alix799-814 in Zn(2+)-bound form. Binding of Ca(2+) to EF3 enables the side chain of Arg125, present in the loop connecting EF3 and EF4, to move enough to make a primary hydrophobic pocket accessible to the critical PPYP motif, which partially overlaps with the GPP motif for the binding of Cep55 (centrosome protein 55 kDa). Based on these results, together with the results of in vitro binding assay with mutant ALG-2 and Alix proteins, we propose a Ca(2+)/EF3-driven arginine switch mechanism for ALG-2 binding to Alix.

PubMed: 18940611
DOI: 10.1016/j.str.2008.07.012

実験手法

X-RAY DIFFRACTION (2.2 Å)