2ZKZ
Crystal structure of the transcriptional repressor PagR of Bacillus anthracis
Summary for 2ZKZ
| Entry DOI | 10.2210/pdb2zkz/pdb |
| Descriptor | Transcriptional repressor pagR (2 entities in total) |
| Functional Keywords | transcriptional repressor, protein-dna, hth motif, dimer, dna-binding, transcription regulation, transcription |
| Biological source | Bacillus anthracis |
| Total number of polymer chains | 4 |
| Total formula weight | 47237.11 |
| Authors | Zhao, H.,Volkov, A.,Veldore, V.H.,Varughese, K.I. (deposition date: 2008-04-01, release date: 2009-04-07, Last modification date: 2024-11-13) |
| Primary citation | Zhao, H.,Volkov, A.,Veldore, V.H.,Hoch, J.A.,Varughese, K.I. Crystal structure of the transcriptional repressor PagR of Bacillus anthracis Microbiology, 156:385-391, 2010 Cited by PubMed Abstract: PagR is a transcriptional repressor in Bacillus anthracis that controls the chromosomal S-layer genes eag and sap, and downregulates the protective antigen pagA gene by direct binding to their promoter regions. The PagR protein sequence is similar to those of members of the ArsR repressor family involved in the repression of arsenate-resistance genes in numerous bacteria. The crystal structure of PagR was solved using multi-wavelength anomalous diffraction (MAD) techniques and was refined with 1.8 A resolution diffraction data. The PagR molecules form dimers, as observed in all SmtB/ArsR repressor family proteins. In the crystal lattice four PagR dimers pack together to form an inactive octamer. Model-building studies suggest that the dimer binds to a DNA duplex with a bend of around 4 degrees. PubMed: 19926656DOI: 10.1099/mic.0.033548-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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