2ZKZ
Crystal structure of the transcriptional repressor PagR of Bacillus anthracis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97969, 1.00344, 1.037 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.010, 73.010, 151.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.870 - 2.000 |
| Rwork | 0.246 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.435 |
| Number of reflections | 36646 | |
| <I/σ(I)> | 45.7 | 2.3 |
| Completeness [%] | 87.0 | 49 |
| Redundancy | 12.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 298 | 0.2M Na Acetate, 1.6M NaH2PO4, 0.4M K2HPO4, pH5.6, hanging drop, temperature 298K |
