2ZB9

Crystal structure of TetR family transcription regulator SCO0332

Summary for 2ZB9

• Entry DOI: 10.2210/pdb2zb9/pdb
• Descriptor: Putative transcriptional regulator (2 entities in total)
• Functional Keywords: transcription regulator, tetr family, helix-turn-helix, dna-binding, transcription regulation, transcription
• Biological source: Streptomyces coelicolor
• Total number of polymer chains: 2
• Total formula weight: 47263.46

Authors

Okada, U.,Kondo, K.,Watanabe, N.,Yao, M.,Tanaka, I. (deposition date: 2007-10-16, release date: 2008-01-29, Last modification date: 2024-03-13)

Primary citation

Okada, U.,Kondo, K.,Hayashi, T.,Watanabe, N.,Yao, M.,Tamura, T.,Tanaka, I.
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor
ACTA CRYSTALLOGR.,SECT.D, 64:198-205, 2008

PubMed Abstract: SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.

PubMed: 18219120
DOI: 10.1107/S0907444907059835

Experimental method

X-RAY DIFFRACTION (2.25 Å)