2ZB9
Crystal structure of TetR family transcription regulator SCO0332
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-03 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 2.29 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.413, 77.778, 57.272 |
| Unit cell angles | 90.00, 104.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| Rwork | 0.195 |
| R-free | 0.24130 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.136 |
| Number of reflections | 21856 | |
| <I/σ(I)> | 46.3 | 13.8 |
| Completeness [%] | 96.4 | 77.3 |
| Redundancy | 7.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% PEG3350, 0.1M HEPES, 0.1M Magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
