2Z77

X-ray crystal structure of RV0760c from Mycobacterium tuberculosis in complex with estradiol-17beta-hemisuccinate

Summary for 2Z77

• Entry DOI: 10.2210/pdb2z77/pdb
• Related: 2A15 2Z76 2Z7A
• Descriptor: Putative steroid isomerase, 4-{[(14beta,17alpha)-3-hydroxyestra-1,3,5(10)-trien-17-yl]oxy}-4-oxobutanoic acid, ACETATE ION, ... (5 entities in total)
• Functional Keywords: alpha+beta conical barrel, complex with estradiol-17beta-hemisuccinate, structural genomics, tb structural genomics consortium, tbsgc, isomerase
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 4
• Total formula weight: 62381.60

Authors

Cherney, M.M.,Garen, C.R.,James, M.N.G.,TB Structural Genomics Consortium (TBSGC) (deposition date: 2007-08-16, release date: 2007-09-18, Last modification date: 2023-08-30)

Primary citation

Cherney, M.M.,Garen, C.R.,James, M.N.
Crystal structure of Mycobacterium tuberculosis Rv0760c at 1.50 A resolution, a structural homolog of Delta(5)-3-ketosteroid isomerase.
Biochim.Biophys.Acta, 1784:1625-1632, 2008

PubMed Abstract: We have determined the X-ray crystal structure of the Mycobacterium tuberculosis (Mtb) gene product encoded by the open reading frame Rv0760c at 1.50 A resolution by single-wavelength anomalous dispersion (SAD) phasing of diffraction data from crystals of the selenomethionine-substituted protein. Refinement against diffraction data from the native protein resulted in R(work)=19.5% and R(free)=21.4%. The X-ray crystal structure shows that the homodimeric Rv0760c polypeptide has an alpha + beta conical barrel fold placing it among many structural neighbors of the nuclear transport factor 2 family (NTF2). This family is highly conserved in terms of structure; however the substrates and individual protein functions are diverse. The structures of native Rv0760c in several different crystal forms and Rv0760c bound to 17beta-estradiol 17-hemisuccinate (EH) have also been solved and analyzed.

PubMed: 18589008
DOI: 10.1016/j.bbapap.2008.05.012

Experimental method

X-RAY DIFFRACTION (2.03 Å)