2Y95
Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation
Summary for 2Y95
| Entry DOI | 10.2210/pdb2y95/pdb |
| NMR Information | BMRB: 16714 |
| Descriptor | 5'-R(*GP*GP*CP*GP*CP*AP*UP*CP*GP*GP*CP*GP*CP*C)-3' (1 entity in total) |
| Functional Keywords | rna, x chromosome inactivation, rna stemloop |
| Biological source | HOMO SAPIENS (HUMAN) |
| Total number of polymer chains | 1 |
| Total formula weight | 4492.74 |
| Authors | Duszczyk, M.M.,Sattler, M. (deposition date: 2011-02-11, release date: 2011-10-26, Last modification date: 2024-06-19) |
| Primary citation | Duszczyk, M.M.,Wutz, A.,Rybin, V.,Sattler, M. The Xist RNA A-Repeat Comprises a Novel Aucg Tetraloop Fold and a Platform for Multimerization. RNA, 17:1973-, 2011 Cited by PubMed Abstract: X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors. PubMed: 21947263DOI: 10.1261/RNA.2747411 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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