2Y6S

Structure of an Ebolavirus-protective antibody in complex with its mucin-domain linear epitope

Summary for 2Y6S

• Entry DOI: 10.2210/pdb2y6s/pdb
• Descriptor: LIGHT CHAIN, HEAVY CHAIN, ENVELOPE GLYCOPROTEIN, ... (4 entities in total)
• Functional Keywords: immune system-viral protein complex, immune system/viral protein
• Biological source: MUS MUSCULUS (HOUSE MOUSE); More
• Cellular location: GP2: Virion membrane; Single-pass type I membrane protein (By similarity). GP1: Virion membrane; Peripheral membrane protein (By similarity). GP2-delta: Secreted (By similarity): 2Y6S
• Total number of polymer chains: 6
• Total formula weight: 96343.22

Authors

Olal, D.O.,Kuehne, A.,Lee, J.E.,Bale, S.,Dye, J.M.,Saphire, E.O. (deposition date: 2011-01-25, release date: 2012-01-11, Last modification date: 2024-10-23)

Primary citation

Olal, D.O.,Kuehne, A.,Bale, S.,Halfmann, P.,Hashiguchi, T.,Fusco, M.L.,Lee, J.E.,King, L.B.,Kawaoka, Y.,Dye, J.M.,Saphire, E.O.
Structure of an Ebola Virus-Protective Antibody in Complex with its Mucin-Domain Linear Epitope.
J.Virol., 86:2809-, 2012

PubMed Abstract: Antibody 14G7 is protective against lethal Ebola virus challenge and recognizes a distinct linear epitope in the prominent mucin-like domain of the Ebola virus glycoprotein GP. The structure of 14G7 in complex with its linear peptide epitope has now been determined to 2.8 Å. The structure shows that this GP sequence forms a tandem β-hairpin structure that binds deeply into a cleft in the antibody-combining site. A key threonine at the apex of one turn is critical for antibody interaction and is conserved among all Ebola viruses. This work provides further insight into the mechanism of protection by antibodies that target the protruding, highly accessible mucin-like domain of Ebola virus and the structural framework for understanding and characterizing candidate immunotherapeutics.

PubMed: 22171276
DOI: 10.1128/JVI.05549-11

Experimental method

X-RAY DIFFRACTION (2.8 Å)