2Y6S
Structure of an Ebolavirus-protective antibody in complex with its mucin-domain linear epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.82, 1.17 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 89.554, 68.376, 92.827 |
| Unit cell angles | 90.00, 112.50, 90.00 |
Refinement procedure
| Resolution | 38.122 - 2.800 |
| R-factor | 0.2386 |
| Rwork | 0.236 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dgg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.300 | 2.800 |
| Rmerge | 0.040 | 0.200 |
| Number of reflections | 46342 | |
| <I/σ(I)> | 19.9 | 2.2 |
| Completeness [%] | 93.9 | 85.1 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PROPANE PH6.5, 20% PEG 3350 |
