2XZO

Upf1 helicase - RNA complex

2XZO の概要

• エントリーDOI: 10.2210/pdb2xzo/pdb
• 関連するPDBエントリー: 2GJK 2GK6 2GK7 2IYK 2WJV 2WJY 2XZP
• 分子名称: REGULATOR OF NONSENSE TRANSCRIPTS 1, 5'-R(*UP*UP*UP*UP*UP*UP*UP)-3', PENTAETHYLENE GLYCOL, ... (7 entities in total)
• 機能のキーワード: hydrolase-rna complex, nmd, rna degradation, allosteric regulation, hydrolase/rna
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm: Q92900
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73420.23

構造登録者

Chakrabarti, S.,Jayachandran, U.,Bonneau, F.,Fiorini, F.,Basquin, C.,Domcke, S.,Le Hir, H.,Conti, E. (登録日: 2010-11-26, 公開日: 2011-03-30, 最終更新日: 2023-12-20)

主引用文献

Chakrabarti, S.,Jayachandran, U.,Bonneau, F.,Fiorini, F.,Basquin, C.,Domcke, S.,Le Hir, H.,Conti, E.
Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and its Regulation by Upf2.
Mol.Cell, 41:693-, 2011

PubMed Abstract: Upf1 is a crucial factor in nonsense-mediated mRNA decay, the eukaryotic surveillance pathway that degrades mRNAs containing premature stop codons. The essential RNA-dependent ATPase activity of Upf1 is triggered by the formation of the surveillance complex with Upf2-Upf3. We report crystal structures of Upf1 in the presence and absence of the CH domain, captured in the transition state with ADP:AlF₄⁻ and RNA. In isolation, Upf1 clamps onto the RNA, enclosing it in a channel formed by both the catalytic and regulatory domains. Upon binding to Upf2, the regulatory CH domain of Upf1 undergoes a large conformational change, causing the catalytic helicase domain to bind RNA less extensively and triggering its helicase activity. Formation of the surveillance complex thus modifies the RNA binding properties and the catalytic activity of Upf1, causing it to switch from an RNA-clamping mode to an RNA-unwinding mode.

PubMed: 21419344
DOI: 10.1016/J.MOLCEL.2011.02.010

実験手法

X-RAY DIFFRACTION (2.395 Å)