2XQC

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE AND ZN

Summary for 2XQC

• Entry DOI: 10.2210/pdb2xqc/pdb
• Related: 2XM3 2XMA 2XO6
• Descriptor: TRANSPOSASE, DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE, 5'-D(TP*TP*GP*AP*TP*GP)-3', ... (5 entities in total)
• Functional Keywords: dna binding protein-dna complex, transposition, mobile element, dna binding protein/dna
• Biological source: DEINOCOCCUS RADIODURANS; More
• Total number of polymer chains: 6
• Total formula weight: 53043.35

Authors

Hickman, A.B.,James, J.A.,Barabas, O.,Pasternak, C.,Ton-Hoang, B.,Chandler, M.,Sommer, S.,Dyda, F. (deposition date: 2010-09-01, release date: 2010-10-13, Last modification date: 2023-12-20)

Primary citation

Hickman, A.B.,James, J.A.,Barabas, O.,Pasternak, C.,Ton-Hoang, B.,Chandler, M.,Sommer, S.,Dyda, F.
DNA Recognition and the Precleavage State During Single-Stranded DNA Transposition in D. Radiodurans.
Embo J., 29:3840-, 2010

PubMed Abstract: Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition. Transposition by one particular family member, ISDra2 from Deinococcus radiodurans, is dramatically stimulated upon massive γ irradiation. We have determined the crystal structures of four ISDra2 transposase/IS end complexes; combined with in vivo activity assays and fluorescence anisotropy binding measurements, these have revealed the molecular basis of strand discrimination and transposase action. The structures also show that previously established structural rules of target site recognition that allow different specific sequences to be targeted are only partially conserved among family members. Furthermore, we have captured a fully assembled active site including the scissile phosphate bound by a divalent metal ion cofactor (Cd²(+)) that supports DNA cleavage. Finally, the observed active site rearrangements when the transposase binds a metal ion in which it is inactive provide a clear rationale for metal ion specificity.

PubMed: 20890269
DOI: 10.1038/EMBOJ.2010.241

Experimental method

X-RAY DIFFRACTION (1.9 Å)