2X50

Crystal structure of Trypanothione reductase from Leishmania infantum in complex with NADPH and silver

2X50 の概要

• エントリーDOI: 10.2210/pdb2x50/pdb
• 関連するPDBエントリー: 2JK6 2W0H
• 分子名称: TRYPANOTHIONE REDUCTASE, FLAVIN-ADENINE DINUCLEOTIDE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (6 entities in total)
• 機能のキーワード: fad, flavoprotein, leishmaniasis, oxidative metabolism, oxidoreductase, redox-active center
• 由来する生物種: LEISHMANIA INFANTUM
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113974.31

構造登録者

Baiocco, P.,Ilari, A.,Colotti, G. (登録日: 2010-02-03, 公開日: 2011-01-12, 最終更新日: 2023-12-20)

主引用文献

Baiocco, P.,Colotti, G.,Ilari, A.
Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leishmania Infantum Proliferation
Acs Med.Chem.Lett., 2:230-, 2011

PubMed Abstract: In Leishmania the glutathione/glutathione reductase eukaryotic redox sys-tem is replaced by the unique trypanothione/trypanothione reductase (TR) system. In vitro, silver is a more effective TR inhibitor than antimony, the first line drug against leishmaniasis in most endemic countries, and its mechanism of inhibition is similar to that of Sb(III). In particular, silver binds with high affinity to the catalytic triad Cys52, Cys57, and His461', thereby inhibiting TR. Here, Ag(0) activity was tested on the promastigote and amastigote stages of Leishmania infantum using a drug-delivery system consisting in Ag(0) nanoparticles encapsulated by ferritin molecules (PfFt-AgNPs). These were able to induce an antiproliferative effect on the parasites at metal concentrations lower than those used with antimony.

PubMed: 24900299
DOI: 10.1021/ML1002629

実験手法

X-RAY DIFFRACTION (3.3 Å)