2WJ8

Respiratory Syncitial Virus RiboNucleoProtein

Summary for 2WJ8

• Entry DOI: 10.2210/pdb2wj8/pdb
• EMDB information: 1622
• Descriptor: NUCLEOPROTEIN, RNA (5'-R(*CP*CP*CP*CP*CP*C)-3'), BORATE ION, ... (4 entities in total)
• Functional Keywords: rna binding protein rna complex, nucleocapsid (n), ribonucleoprotein, respiratory syncytial virus (rsv), viral nucleoprotein, template-like assembly, rna, virion, complex, cytoplasm, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• Biological source: HUMAN RESPIRATORY SYNCYTIAL VIRUS A STRAIN LONG; More
• Total number of polymer chains: 40
• Total formula weight: 912929.34

Authors

Tawar, R.G.,Duquerroy, S.,Vonrhein, C.,Varela, P.F.,Damier-Piolle, L.,Castagne, N.,MacLellan, K.,Bedouelle, H.,Bricogne, G.,Bhella, D.,Eleouet, J.,Rey, F.A. (deposition date: 2009-05-25, release date: 2009-12-08, Last modification date: 2024-05-01)

Primary citation

Tawar, R.G.,Duquerroy, S.,Vonrhein, C.,Varela, P.F.,Damier-Piolle, L.,Castagne, N.,Maclellan, K.,Bedouelle, H.,Bricogne, G.,Bhella, D.,Eleouet, J.,Rey, F.A.
Crystal Structure of a Nucleocapsid-Like Nucleoprotein-RNA Complex of Respiratory Syncytial Virus
Science, 326:1279-, 2009

PubMed Abstract: The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of this RNA virus, we determined the three-dimensional (3D) crystal structure at 3.3 A resolution of a decameric, annular ribonucleoprotein complex of the RSV nucleoprotein (N) bound to RNA. This complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral RNA synthesis. The RNA wraps around the protein ring, with seven nucleotides contacting each N subunit, alternating rows of four and three stacked bases that are exposed and buried within a protein groove, respectively. Combined with electron microscopy data, this structure provides a detailed model for the RSV nucleocapsid, in which the bases are accessible for readout by the viral polymerase. Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting.

PubMed: 19965480
DOI: 10.1126/SCIENCE.1177634

Experimental method

X-RAY DIFFRACTION (3.29 Å)