2WC1
Three-dimensional Structure of the Nitrogen Fixation Flavodoxin (NifF) from Rhodobacter capsulatus at 2.2 A
Summary for 2WC1
| Entry DOI | 10.2210/pdb2wc1/pdb |
| Descriptor | FLAVODOXIN, FLAVIN MONONUCLEOTIDE (3 entities in total) |
| Functional Keywords | electron transport, flavoprotein |
| Biological source | RHODOBACTER CAPSULATUS |
| Total number of polymer chains | 1 |
| Total formula weight | 20290.75 |
| Authors | Perez-Dorado, I.,Bittel, C.,Hermoso, J.A.,Cortez, N.,Carrillo, N. (deposition date: 2009-03-06, release date: 2010-04-21, Last modification date: 2023-12-13) |
| Primary citation | Perez-Dorado, I.,Bortolotti, A.,Cortez, N.,Hermoso, J.A. Structural and Phylogenetic Analysis of Rhodobacter Capsulatus Niff: Uncovering General Features of Nitrogen-Fixation (Nif)-Flavodoxins. Int.J.Mol.Sci., 14:1152-, 2013 Cited by PubMed Abstract: Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50's loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as "short-chain" with the firmicutes flavodoxins and the "long-chain" with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase. PubMed: 23303276DOI: 10.3390/IJMS14011152 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.17 Å) |
Structure validation
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