2WC1
Three-dimensional Structure of the Nitrogen Fixation Flavodoxin (NifF) from Rhodobacter capsulatus at 2.2 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.400, 66.400, 121.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.660 - 2.170 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.700 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 24830 | |
| <I/σ(I)> | 26 | 8.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 22.4 | 22.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
