2WAQ
The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase
Summary for 2WAQ
| Entry DOI | 10.2210/pdb2waq/pdb |
| Related | 2WB1 |
| Descriptor | DNA-DIRECTED RNA POLYMERASE RPO1N SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO11 SUBUNIT, DNA-DIRECTED RNA POLYMERASE RPO10 SUBUNIT, ... (16 entities in total) |
| Functional Keywords | multi-subunit, rna polymerase, transcription |
| Biological source | SULFOLOBUS SHIBATAE More |
| Total number of polymer chains | 13 |
| Total formula weight | 406595.40 |
| Authors | Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G.A. (deposition date: 2009-02-11, release date: 2009-05-19, Last modification date: 2024-11-13) |
| Primary citation | Korkhin, Y.,Unligil, U.M.,Littlefield, O.,Nelson, P.J.,Stuart, D.I.,Sigler, P.B.,Bell, S.D.,Abrescia, N.G. Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure. Plos Biol., 7:e1000102-e1000102, 2009 Cited by PubMed Abstract: The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble. PubMed: 19419240DOI: 10.1371/journal.pbio.1000102 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.35 Å) |
Structure validation
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