2WAQ
The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 193.501, 212.620, 128.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.780 - 3.350 |
R-factor | 0.274 |
Rwork | 0.271 |
R-free | 0.34100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1EN0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 59401 | |
<I/σ(I)> | 8.5 | 1.7 |
Completeness [%] | 77.0 | 44.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 7MG/ML ENZYME, 150MM KCL, 100MM SRCL2, 1MM ZNCL2, 100MM CACODYLATE PH 6.5, 12% PEG MME 5K, 5% GLYCEROL |