2VOO

Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer UUUUUUUU

Summary for 2VOO

• Entry DOI: 10.2210/pdb2voo/pdb
• Related: 1OWX 1S79 1S7A 1YTY 1ZH5 2VOD 2VON 2VOP
• Descriptor: LUPUS LA PROTEIN, 5'-R(*UP*UP*UP*UP*UP*UP*UP)-3' (3 entities in total)
• Functional Keywords: rna-binding protein, rna recognition motif, systemic lupus erythematosus, phosphoprotein, rna maturation, rna binding protein, nucleus, la motif, rna-binding, polymorphism
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus (Probable): P05455
• Total number of polymer chains: 4
• Total formula weight: 49256.02

Authors

Kotik-Kogan, O.,Valentine, E.R.,Sanfelice, D.,Conte, M.R.,Curry, S. (deposition date: 2008-02-19, release date: 2008-05-06, Last modification date: 2023-12-13)

Primary citation

Kotik-Kogan, O.,Valentine, E.R.,Sanfelice, D.,Conte, M.R.,Curry, S.
Structural Analysis Reveals Conformational Plasticity in the Recognition of RNA 3' Ends by the Human La Protein.
Structure, 16:852-, 2008

PubMed Abstract: The eukaryotic La protein recognizes the 3' poly(U) sequences of nascent RNA polymerase III transcripts to assist folding and maturation. The 3' ends of such RNAs are bound by the N-terminal domain of La (LaNTD). We have solved the crystal structures of four LaNTD:RNA complexes, each containing a different single-stranded RNA oligomer, and compared them to the structure of a previously published LaNTD:RNA complex containing partially duplex RNA. The presence of purely single-stranded RNA in the binding pocket at the interface between the La motif and RRM domains allows significantly closer contact with the 3' end of the RNA. Comparison of the different LaNTD:RNA complexes identifies a conserved set of interactions with the last two nucleotides at the 3' end of the RNA ligand that are key to binding. Strikingly, we also observe two alternative conformations of bound ssRNA, indicative of an unexpected degree of plasticity in the modes of RNA binding.

PubMed: 18547518
DOI: 10.1016/J.STR.2008.02.021

Experimental method

X-RAY DIFFRACTION (1.8 Å)