2VL8

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF LETHAL TOXIN FROM CLOSTRIDIUM SORDELLII IN COMPLEX WITH UDP, CASTANOSPERMINE AND CALCIUM ION

Summary for 2VL8

Entry DOI	10.2210/pdb2vl8/pdb
Related	2VKD 2VKH
Descriptor	CYTOTOXIN L, URIDINE-5'-DIPHOSPHATE, CASTANOSPERMINE, ... (5 entities in total)
Functional Keywords	toxin, glycosyltransferase
Biological source	CLOSTRIDIUM SORDELLII
Total number of polymer chains	3
Total formula weight	192800.67
Authors	Jank, T.,Ziegler, M.O.P.,Schulz, G.E.,Aktories, K. (deposition date: 2008-01-09, release date: 2008-06-17, Last modification date: 2024-05-08)
Primary citation	Jank, T.,Ziegler, M.O.P.,Schulz, G.E.,Aktories, K. Inhibition of the Glucosyltransferase Activity of Clostridial Rho/Ras-Glucosylating Toxins by Castanospermine. FEBS Lett., 582:2277-, 2008 Cited by PubMed Abstract: Castanospermine was identified as an inhibitor of the Rho/Ras-glucosylating Clostridium sordellii lethal toxin and Clostridium difficile toxin B. Microinjection of castanospermine into embryonic bovine lung cells prevented the cytotoxic effects of toxins. The crystal structure of the glucosyltransferase domain of C. sordellii lethal toxin in complex with castanospermine, UDP and a calcium ion was solved at a resolution of 2.3A. The inhibitor binds in a conformation that brings its four hydroxyl groups and its N-atom almost exactly in the positions of the four hydroxyls and of the ring oxygen of the glucosyl moiety of UDP-glucose, respectively. PubMed: 18505687 DOI: 10.1016/J.FEBSLET.2008.05.025 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.31 Å)

Structure validation