2VE7

Crystal structure of a bonsai version of the human Ndc80 complex

2VE7 の概要

• エントリーDOI: 10.2210/pdb2ve7/pdb
• 分子名称: KINETOCHORE PROTEIN HEC1, KINETOCHORE PROTEIN SPC25, KINETOCHORE PROTEIN NUF2, KINETOCHORE PROTEIN SPC24, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: mitosis, centromere, cell cycle, microtubule, kinetochore, cell division, calponin homology
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: Q9HBM1 Q8NBT2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131061.84

構造登録者

Ciferri, C.,Pasqualato, S.,Dos Reis, G.,Screpanti, E.,Maiolica, A.,Polka, J.,De Luca, J.G.,De Wulf, P.,Salek, M.,Rappsilber, J.,Moores, C.A.,Salmon, E.D.,Musacchio, A. (登録日: 2007-10-17, 公開日: 2008-05-13, 最終更新日: 2024-06-19)

主引用文献

Ciferri, C.,Pasqualato, S.,Screpanti, E.,Varetti, G.,Santaguida, S.,Dos Reis, G.,Maiolica, A.,Polka, J.,De Luca, J.G.,De Wulf, P.,Salek, M.,Rappsilber, J.,Moores, C.A.,Salmon, E.D.,Musacchio, A.
Implications for Kinetochore-Microtubule Attachment from the Structure of an Engineered Ndc80 Complex
Cell(Cambridge,Mass.), 133:427-, 2008

PubMed Abstract: Kinetochores are proteinaceous assemblies that mediate the interaction of chromosomes with the mitotic spindle. The 180 kDa Ndc80 complex is a direct point of contact between kinetochores and microtubules. Its four subunits contain coiled coils and form an elongated rod structure with functional globular domains at either end. We crystallized an engineered "bonsai" Ndc80 complex containing a shortened rod domain but retaining the globular domains required for kinetochore localization and microtubule binding. The structure reveals a microtubule-binding interface containing a pair of tightly interacting calponin-homology (CH) domains with a previously unknown arrangement. The interaction with microtubules is cooperative and predominantly electrostatic. It involves positive charges in the CH domains and in the N-terminal tail of the Ndc80 subunit and negative charges in tubulin C-terminal tails and is regulated by the Aurora B kinase. We discuss our results with reference to current models of kinetochore-microtubule attachment and centromere organization.

PubMed: 18455984
DOI: 10.1016/J.CELL.2008.03.020

実験手法

X-RAY DIFFRACTION (2.88 Å)