2RGP

Structure of EGFR in complex with hydrazone, a potent dual inhibitor

Summary for 2RGP

• Entry DOI: 10.2210/pdb2rgp/pdb
• Descriptor: Epidermal growth factor receptor, PHOSPHATE ION, N-[1-(3-fluorobenzyl)-1H-indazol-5-yl]-5-[(piperidin-1-ylamino)methyl]pyrimidine-4,6-diamine, ... (4 entities in total)
• Functional Keywords: kinase domain, alternative splicing, anti-oncogene, atp-binding, cell cycle, disease mutation, glycoprotein, membrane, nucleotide-binding, phosphoprotein, polymorphism, receptor, secreted, transferase, transmembrane, tyrosine-protein kinase, ubl conjugation
• Biological source: Homo sapiens (Human)
• Cellular location: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted: P00533
• Total number of polymer chains: 1
• Total formula weight: 36707.27

Authors

Abad, M.C. (deposition date: 2007-10-04, release date: 2008-08-26, Last modification date: 2023-08-30)

Primary citation

Xu, G.,Abad, M.C.,Connolly, P.J.,Neeper, M.P.,Struble, G.T.,Springer, B.A.,Emanuel, S.L.,Pandey, N.,Gruninger, R.H.,Adams, M.,Moreno-Mazza, S.,Fuentes-Pesquera, A.R.,Middleton, S.A.
4-Amino-6-arylamino-pyrimidine-5-carbaldehyde hydrazones as potent ErbB-2/EGFR dual kinase inhibitors.
Bioorg.Med.Chem.Lett., 18:4615-4619, 2008

PubMed Abstract: Members of a novel class of 4-amino-6-arylamino-pyrimidine-5-carbaldehyde hydrazones were identified as potent dual ErbB-2/EGFR kinase inhibitors using concept-guided design approach. These compounds inhibited the growth of ErbB-2 over-expressing human tumor cell lines (BT474, N87, and SK-BR-3) in vitro. Compound 15 emerged as a key lead and showed significant ability to inhibit growth factor-induced receptor phosphorylation in SK-BR-3 cells (IC(50)=54 nM) and cellular proliferation in vitro (IC(50)=14, 58, and 58 nM for BT474, N87, and SK-BR-3 respectively). The X-ray co-crystal structure of EGFR with a close analog (17) was determined and validated our design rationale.

PubMed: 18653333
DOI: 10.1016/j.bmcl.2008.07.020

Experimental method

X-RAY DIFFRACTION (2 Å)