2R7G

Structure of the retinoblastoma protein pocket domain in complex with adenovirus E1A CR1 domain

2R7G の概要

• エントリーDOI: 10.2210/pdb2r7g/pdb
• 分子名称: Retinoblastoma-associated protein, Early E1A 32 kDa protein, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: retinoblastoma protein, e1a, e2f displacement, transcription repressor, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P06400; Host nucleus : P03255
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 85243.78

構造登録者

Liu, X.,Marmorstein, R. (登録日: 2007-09-07, 公開日: 2007-10-02, 最終更新日: 2024-02-21)

主引用文献

Liu, X.,Marmorstein, R.
Structure of the retinoblastoma protein bound to adenovirus E1A reveals the molecular basis for viral oncoprotein inactivation of a tumor suppressor
Genes Dev., 21:2711-2716, 2007

PubMed Abstract: The adenovirus (Ad) E1A (Ad-E1A) oncoprotein mediates cell transformation, in part, by displacing E2F transcription factors from the retinoblastoma protein (pRb) tumor suppressor. In this study we determined the crystal structure of the pRb pocket domain in complex with conserved region 1 (CR1) of Ad5-E1A. The structure and accompanying biochemical studies reveal that E1A-CR1 binds at the interface of the A and B cyclin folds of the pRb pocket domain, and that both E1A-CR1 and the E2F transactivation domain use similar conserved nonpolar residues to engage overlapping sites on pRb, implicating a novel molecular mechanism for pRb inactivation by a viral oncoprotein.

PubMed: 17974914
DOI: 10.1101/gad.1590607

実験手法

X-RAY DIFFRACTION (1.671 Å)