2R29

Neutralization of dengue virus by a serotype cross-reactive antibody elucidated by cryoelectron microscopy and x-ray crystallography

Summary for 2R29

• Entry DOI: 10.2210/pdb2r29/pdb
• Descriptor: Envelope protein E, Heavy chain of Fab 1A1D-2, Light chain of Fab 1A1D-2, ... (4 entities in total)
• Functional Keywords: fab-antigen complex, atp-binding, capsid protein, cleavage on pair of basic residues, endoplasmic reticulum, envelope protein, glycoprotein, helicase, hydrolase, membrane, metal-binding, multifunctional enzyme, nucleotide-binding, nucleotidyltransferase, nucleus, phosphorylation, protease, ribonucleoprotein, rna replication, rna-binding, rna-directed rna polymerase, secreted, serine protease, transcription, transcription regulation, transferase, transmembrane, viral nucleoprotein, virion, viral protein-immune system complex, viral protein/immune system
• Biological source: Dengue virus 2 Thailand/16681/84; More
• Cellular location: Capsid protein C: Virion . Peptide pr: Secreted . Small envelope protein M: Virion membrane ; Multi-pass membrane protein . Envelope protein E: Virion membrane ; Multi-pass membrane protein . Non-structural protein 1: Secreted . Non-structural protein 2A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side: P14340
• Total number of polymer chains: 3
• Total formula weight: 57970.86

Authors

Lok, S.M.,Kostyuchenko, V.K.,Nybakken, G.E.,Holdaway, H.A.,Battisti, A.J.,Sukupolvi-petty, S.,Sedlak, D.,Fremont, D.H.,Chipman, P.R.,Roehrig, J.T.,Diamond, M.S.,Kuhn, R.J.,Rossmann, M.G. (deposition date: 2007-08-24, release date: 2007-12-25, Last modification date: 2018-01-24)

• Primary citation: Lok, S.M.,Kostyuchenko, V.,Nybakken, G.E.,Holdaway, H.A.,Battisti, A.J.,Sukupolvi-Petty, S.,Sedlak, D.,Fremont, D.H.,Chipman, P.R.,Roehrig, J.T.,Diamond, M.S.,Kuhn, R.J.,Rossmann, M.G.; Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins.; Nat.Struct.Mol.Biol., 15:312-317, 2008; PubMed: 18264114; DOI: 10.1038/nsmb.1382

Experimental method

X-RAY DIFFRACTION (3 Å)