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2R1C

Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex

Summary for 2R1C
Entry DOI10.2210/pdb2r1c/pdb
Related1JOS 2DYJ 2R1G
EMDB information1413
DescriptorRibosome-binding factor A (1 entity in total)
Functional Keywordshelix-kink-helix, kh domain, rrna processing, rna binding protein
Biological sourceThermus thermophilus
Total number of polymer chains1
Total formula weight10875.71
Authors
Datta, P.P.,Wilson, D.N.,Kawazoe, M.,Swami, N.K.,Kaminishi, T.,Sharma, M.R.,Booth, T.M.,Takemoto, C.,Fucini, P.,Yokoyama, S.,Agrawal, R.K. (deposition date: 2007-08-22, release date: 2008-03-18, Last modification date: 2024-03-13)
Primary citationDatta, P.P.,Wilson, D.N.,Kawazoe, M.,Swami, N.K.,Kaminishi, T.,Sharma, M.R.,Booth, T.M.,Takemoto, C.,Fucini, P.,Yokoyama, S.,Agrawal, R.K.
Structural aspects of RbfA action during small ribosomal subunit assembly.
Mol.Cell, 28:434-445, 2007
Cited by
PubMed Abstract: Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock.
PubMed: 17996707
DOI: 10.1016/j.molcel.2007.08.026
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (12.5 Å)
Structure validation

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