2DYJ
Crystal structure of ribosome-binding factor A from Thermus thermophilus HB8
Summary for 2DYJ
| Entry DOI | 10.2210/pdb2dyj/pdb |
| Descriptor | Ribosome-binding factor A (2 entities in total) |
| Functional Keywords | 16s rrna processing, 17s rna, kh domain, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, ribosomal protein |
| Biological source | Thermus thermophilus |
| Total number of polymer chains | 2 |
| Total formula weight | 21751.42 |
| Authors | Kawazoe, M.,Takemoto, C.,Nakayama-Ushikoshi, R.,Terada, T.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2006-09-14, release date: 2007-03-14, Last modification date: 2023-10-25) |
| Primary citation | Datta, P.P.,Wilson, D.N.,Kawazoe, M.,Swami, N.K.,Kaminishi, T.,Sharma, M.R.,Booth, T.M.,Takemoto, C.,Fucini, P.,Yokoyama, S.,Agrawal, R.K. Structural aspects of RbfA action during small ribosomal subunit assembly. Mol.Cell, 28:434-445, 2007 Cited by PubMed Abstract: Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock. PubMed: 17996707DOI: 10.1016/j.molcel.2007.08.026 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.84 Å) |
Structure validation
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