2QUW
Hammerhead Ribozyme G12A mutant after cleavage
Summary for 2QUW
| Entry DOI | 10.2210/pdb2quw/pdb |
| Related | 2GOZ 2QUS |
| Descriptor | Hammerhead ribozyme, cleaved fragment, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | hammerhead ribozyme, ribozyme, catalytic rna, ribozyme-product complex, rna |
| Total number of polymer chains | 4 |
| Total formula weight | 45255.53 |
| Authors | Chi, Y.I.,Scott, W.G.,Kim, S.H. (deposition date: 2007-08-06, release date: 2008-07-15, Last modification date: 2024-02-21) |
| Primary citation | Chi, Y.I.,Martick, M.,Lares, M.,Kim, R.,Scott, W.G.,Kim, S.H. Capturing hammerhead ribozyme structures in action by modulating general base catalysis. Plos Biol., 6:e234-e234, 2008 Cited by PubMed Abstract: We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures. PubMed: 18834200DOI: 10.1371/journal.pbio.0060234 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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