2QUW
Hammerhead Ribozyme G12A mutant after cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.030, 53.140, 72.050 |
| Unit cell angles | 74.24, 81.37, 75.65 |
Refinement procedure
| Resolution | 19.460 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.199 |
| R-free | 0.26200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.762 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.460 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.080 |
| Number of reflections | 15733 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 89.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
