2QRT

Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C, beta-2microglobulin, and ovalbumin-derived peptide.

Summary for 2QRT

• Entry DOI: 10.2210/pdb2qrt/pdb
• Related: 2QRI 2QRS
• Descriptor: H-2 class I histocompatibility antigen K-B alpha chain, Beta-2 microglobulin, ovalbumin-derived peptide (2 entities in total)
• Functional Keywords: mhc-i, ova, single chain mhc-i, glycoprotein, immune response, membrane, mhc i, transmembrane, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 94104.86

Authors

Mitaksov, V.E.,Fremont, D.H. (deposition date: 2007-07-29, release date: 2007-11-06, Last modification date: 2024-11-20)

Primary citation

Mitaksov, V.,Truscott, S.M.,Lybarger, L.,Connolly, J.M.,Hansen, T.H.,Fremont, D.H.
Structural engineering of pMHC reagents for T cell vaccines and diagnostics.
Chem.Biol., 14:909-922, 2007

PubMed Abstract: MHC class I peptide complexes (pMHC) are routinely used to enumerate T cell populations and are currently being evaluated as vaccines to tumors and specific pathogens. Herein, we describe the structures of three generations of single-chain pMHC progressively designed for the optimal presentation of covalently associated epitopes. Our ultimate design employs a versatile disulfide trap between an invariant MHC residue and a short C-terminal peptide extension. This general strategy is nondisruptive of native pMHC conformation and T cell receptor engagement. Indeed, cell-surface-expressed MHC complexes with disulfide-trapped epitopes are refractory to peptide exchange, suggesting they will make safe and effective vaccines. Furthermore, we find that disulfide-trap stabilized, recombinant pMHC reagents reliably detect polyclonal CD8 T cell populations as proficiently as conventional reagents and are thus well suited to monitor or modulate immune responses during pathogenesis.

PubMed: 17719490
DOI: 10.1016/j.chembiol.2007.07.010

Experimental method

X-RAY DIFFRACTION (1.8 Å)