2QMR

Karyopherin beta2/transportin

2QMR の概要

• エントリーDOI: 10.2210/pdb2qmr/pdb
• 関連するPDBエントリー: 1qbk 2h4m 2ot8
• 分子名称: Transportin-1 (1 entity in total)
• 機能のキーワード: heat repeat, cytoplasm, host-virus interaction, nucleus, polymorphism, protein transport, transport, transport protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q92973
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 405633.75

構造登録者

Cansizoglu, A.E.,Chook, Y.M. (登録日: 2007-07-16, 公開日: 2007-10-30, 最終更新日: 2023-08-30)

主引用文献

Cansizoglu, A.E.,Chook, Y.M.
Conformational heterogeneity of karyopherin beta2 is segmental
Structure, 15:1431-1441, 2007

PubMed Abstract: Karyopherinbeta2 (Kap beta2) or transportin imports numerous RNA binding proteins into the nucleus. Kap beta2 binds substrates in the cytoplasm and targets them through the nuclear pore complex, where RanGTP dissociates them in the nucleus. Here we report the 3.0 A crystal structure of unliganded Kap beta2, which consists of a superhelix of 20 HEAT repeats. Together with previously reported structures of NLS and Ran complexes, this structure provides understanding of conformational heterogeneity that accompanies ligand binding. The Kap beta2 superhelix is divided into three major segments. Two of them (HEAT repeats 9-13 and 14-18), which constitute the substrate binding site, are rigid elements that rotate relative to each other about a flexible hinge. The third (HEAT repeats 1-8), which constitutes the Ran binding site, exhibits conformational changes throughout its length. An analogous segmental architecture is also observed in Importin beta, suggesting that it is functionally significant and may be conserved in other import karyopherins.

PubMed: 17997969
DOI: 10.1016/j.str.2007.09.009

実験手法

X-RAY DIFFRACTION (3 Å)