2QMR
Karyopherin beta2/transportin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 129.338, 168.892, 140.958 |
Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.26185 |
Rwork | 0.261 |
R-free | 0.28575 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h4m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.124 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.950 |
Rmerge | 0.083 |
Number of reflections | 126673 |
<I/σ(I)> | 24 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.2 M potassium formate, 0.1 M HEPES pH 7.5 and 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |