2OT8

Karyopherin Beta2/Transportin-hnRNPM NLS Complex

Summary for 2OT8

• Entry DOI: 10.2210/pdb2ot8/pdb
• Related: 1qbk 2h4m
• Descriptor: Transportin-1, Heterogeneous nuclear ribonucleoprotein M (2 entities in total)
• Functional Keywords: heat repeat, nuclear import complex, karyopherin, transport protein
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: Q92973; Nucleus, nucleolus {ECO:0000269|Ref: P52272
• Total number of polymer chains: 4
• Total formula weight: 200491.46

Authors

Cansizoglu, A.E.,Chook, Y.M. (deposition date: 2007-02-07, release date: 2007-04-10, Last modification date: 2024-10-30)

Primary citation

Cansizoglu, A.E.,Lee, B.J.,Zhang, Z.C.,Fontoura, B.M.,Chook, Y.M.
Structure-based design of a pathway-specific nuclear import inhibitor.
Nat.Struct.Mol.Biol., 14:452-454, 2007

PubMed Abstract: Kapbeta2 (also called transportin) recognizes PY nuclear localization signal (NLS), a new class of NLS with a R/H/Kx((2-5))PY motif. Here we show that Kapbeta2 complexes containing hydrophobic and basic PY-NLSs, as classified by the composition of an additional N-terminal motif, converge in structure only at consensus motifs, which explains ligand diversity. On the basis of these data and complementary biochemical analyses, we designed a Kapbeta2-specific nuclear import inhibitor, M9M.

PubMed: 17435768
DOI: 10.1038/nsmb1229

Experimental method

X-RAY DIFFRACTION (3.1 Å)