2QI0
Crystal structure of protease inhibitor, MIT-1-KK80 in complex with wild type HIV-1 protease
Summary for 2QI0
Entry DOI | 10.2210/pdb2qi0/pdb |
Related | 2QHY 2QHZ 2QI1 2QI3 2QI4 2QI5 2QI6 2QI7 |
Descriptor | Protease, PHOSPHATE ION, N-[(1S,2R)-1-BENZYL-2-HYDROXY-3-{[(3-METHOXYPHENYL)SULFONYL](2-THIENYLMETHYL)AMINO}PROPYL]-3-FLUORO-2-METHYLBENZAMIDE, ... (4 entities in total) |
Functional Keywords | drug design, hiv-1 protease, protease inhibitors, hydrolase |
Biological source | Human immunodeficiency virus 1 |
Total number of polymer chains | 2 |
Total formula weight | 22309.26 |
Authors | Schiffer, C.A.,Nalam, M.N.L. (deposition date: 2007-07-03, release date: 2008-04-22, Last modification date: 2023-08-30) |
Primary citation | Altman, M.D.,Ali, A.,Reddy, G.S.,Nalam, M.N.,Anjum, S.G.,Cao, H.,Chellappan, S.,Kairys, V.,Fernandes, M.X.,Gilson, M.K.,Schiffer, C.A.,Rana, T.M.,Tidor, B. HIV-1 protease inhibitors from inverse design in the substrate envelope exhibit subnanomolar binding to drug-resistant variants. J.Am.Chem.Soc., 130:6099-6113, 2008 Cited by PubMed Abstract: The acquisition of drug-resistant mutations by infectious pathogens remains a pressing health concern, and the development of strategies to combat this threat is a priority. Here we have applied a general strategy, inverse design using the substrate envelope, to develop inhibitors of HIV-1 protease. Structure-based computation was used to design inhibitors predicted to stay within a consensus substrate volume in the binding site. Two rounds of design, synthesis, experimental testing, and structural analysis were carried out, resulting in a total of 51 compounds. Improvements in design methodology led to a roughly 1000-fold affinity enhancement to a wild-type protease for the best binders, from a Ki of 30-50 nM in round one to below 100 pM in round two. Crystal structures of a subset of complexes revealed a binding mode similar to each design that respected the substrate envelope in nearly all cases. All four best binders from round one exhibited broad specificity against a clinically relevant panel of drug-resistant HIV-1 protease variants, losing no more than 6-13-fold affinity relative to wild type. Testing a subset of second-round compounds against the panel of resistant variants revealed three classes of inhibitors: robust binders (maximum affinity loss of 14-16-fold), moderate binders (35-80-fold), and susceptible binders (greater than 100-fold). Although for especially high-affinity inhibitors additional factors may also be important, overall, these results suggest that designing inhibitors using the substrate envelope may be a useful strategy in the development of therapeutics with low susceptibility to resistance. PubMed: 18412349DOI: 10.1021/ja076558p PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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