2QFA

Crystal structure of a Survivin-Borealin-INCENP core complex

2QFA の概要

• エントリーDOI: 10.2210/pdb2qfa/pdb
• 分子名称: Baculoviral IAP repeat-containing protein 5, Borealin, Inner centromere protein, ... (6 entities in total)
• 機能のキーワード: three-helical-bundle, long helix, protein complex, alternative splicing, apoptosis, cell cycle, cell division, centromere, chromosomal protein, cytoplasm, metal-binding, mitosis, nucleus, phosphorylation, polymorphism, protease inhibitor, thiol protease inhibitor, zinc, coiled coil, microtubule, cell cycle-cell cycle-cell cycle complex, cell cycle/cell cycle/cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: O15392; Nucleus, nucleolus: Q53HL2; Chromosome, centromere: Q9NQS7
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29870.69

構造登録者

Jeyaprakash, A.A.,Klein, U.R.,Lindner, D.,Ebert, J.,Nigg, E.A.,Conti, E. (登録日: 2007-06-27, 公開日: 2007-11-06, 最終更新日: 2024-02-21)

主引用文献

Jeyaprakash, A.A.,Klein, U.R.,Lindner, D.,Ebert, J.,Nigg, E.A.,Conti, E.
Structure of a Survivin-Borealin-INCENP Core Complex Reveals How Chromosomal Passengers Travel Together.
Cell(Cambridge,Mass.), 131:271-285, 2007

PubMed Abstract: The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization.

PubMed: 17956729
DOI: 10.1016/j.cell.2007.07.045

実験手法

X-RAY DIFFRACTION (1.4 Å)