2Q7K

The Androgen Receptor Prostate Cancer Mutant H874Y Ligand Binding Domain Bound with Testosterone and an AR 20-30 Peptide

Summary for 2Q7K

• Entry DOI: 10.2210/pdb2q7k/pdb
• Descriptor: Androgen receptor, SULFATE ION, TESTOSTERONE, ... (6 entities in total)
• Functional Keywords: androgen receptor prostate cancer mutant h874y ligand binding domain, testoseterone, n-terminal ar peptide, hormone
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P10275 P10275
• Total number of polymer chains: 2
• Total formula weight: 31872.18

Authors

Gampe, R.T. (deposition date: 2007-06-07, release date: 2007-07-24, Last modification date: 2024-02-21)

Primary citation

Askew, E.B.,Gampe, R.T.,Stanley, T.B.,Faggart, J.L.,Wilson, E.M.
Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone.
J.Biol.Chem., 282:25801-25816, 2007

PubMed Abstract: The androgen receptor (AR) is transcriptionally activated by high affinity binding of testosterone (T) or its 5alpha-reduced metabolite, dihydrotestosterone (DHT), a more potent androgen required for male reproductive tract development. The molecular basis for the weaker activity of T was investigated by determining T-bound ligand binding domain crystal structures of wild-type AR and a prostate cancer somatic mutant complexed with the AR FXXLF or coactivator LXXLL peptide. Nearly identical interactions of T and DHT in the AR ligand binding pocket correlate with similar rates of dissociation from an AR fragment containing the ligand binding domain. However, T induces weaker AR FXXLF and coactivator LXXLL motif interactions at activation function 2 (AF2). Less effective FXXLF motif binding to AF2 accounts for faster T dissociation from full-length AR. T can nevertheless acquire DHT-like activity through an AR helix-10 H874Y prostate cancer mutation. The Tyr-874 mutant side chain mediates a new hydrogen bonding scheme from exterior helix-10 to backbone protein core helix-4 residue Tyr-739 to rescue T-induced AR activity by improving AF2 binding of FXXLF and LXXLL motifs. Greater AR AF2 activity by improved core helix interactions is supported by the effects of melanoma antigen gene protein-11, an AR coregulator that binds the AR FXXLF motif and targets AF2 for activation. We conclude that T is a weaker androgen than DHT because of less favorable T-dependent AR FXXLF and coactivator LXXLL motif interactions at AF2.

PubMed: 17591767
DOI: 10.1074/jbc.M703268200

Experimental method

X-RAY DIFFRACTION (1.8 Å)