2PRG

LIGAND-BINDING DOMAIN OF THE HUMAN PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA

2PRG の概要

• エントリーDOI: 10.2210/pdb2prg/pdb
• 分子名称: PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA, NUCLEAR RECEPTOR COACTIVATOR SRC-1, 2,4-THIAZOLIDIINEDIONE, 5-[[4-[2-(METHYL-2-PYRIDINYLAMINO)ETHOXY]PHENYL]METHYL]-(9CL), ... (4 entities in total)
• 機能のキーワード: complex (thiazolidinedione-receptor), ligand-binding domain, nuclear receptor, apo, transcription factor, orphan receptor, complex (thiazolidinedione-receptor) complex, complex (thiazolidinedione/receptor)
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 72105.12

構造登録者

Nolte, R.T.,Wisely, G.B.,Milburn, M.V. (登録日: 1998-08-14, 公開日: 1999-07-19, 最終更新日: 2024-05-22)

主引用文献

Nolte, R.T.,Wisely, G.B.,Westin, S.,Cobb, J.E.,Lambert, M.H.,Kurokawa, R.,Rosenfeld, M.G.,Willson, T.M.,Glass, C.K.,Milburn, M.V.
Ligand binding and co-activator assembly of the peroxisome proliferator-activated receptor-gamma.
Nature, 395:137-143, 1998

PubMed Abstract: The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is a ligand-dependent transcription factor that is important in adipocyte differentiation and glucose homeostasis and which depends on interactions with co-activators, including steroid receptor co-activating factor-1 (SRC-1). Here we present the X-ray crystal structure of the human apo-PPAR-gamma ligand-binding domain (LBD), at 2.2 A resolution; this structure reveals a large binding pocket, which may explain the diversity of ligands for PPAR-gamma. We also describe the ternary complex containing the PPAR-gamma LBD, the antidiabetic ligand rosiglitazone (BRL49653), and 88 amino acids of human SRC-1 at 2.3 A resolution. Glutamate and lysine residues that are highly conserved in LBDs of nuclear receptors form a 'charge clamp' that contacts backbone atoms of the LXXLL helices of SRC-1. These results, together with the observation that two consecutive LXXLL motifs of SRC-1 make identical contacts with both subunits of a PPAR-gamma homodimer, suggest a general mechanism for the assembly of nuclear receptors with co-activators.

PubMed: 9744270
DOI: 10.1038/25931

実験手法

X-RAY DIFFRACTION (2.3 Å)