2PK8

Crystal structure of an uncharacterized protein PF0899 from Pyrococcus furiosus

Replaces:  1SHE

Summary for 2PK8

• Entry DOI: 10.2210/pdb2pk8/pdb
• Descriptor: Uncharacterized protein PF0899, GOLD (I) CYANIDE ION (3 entities in total)
• Functional Keywords: pf0899, structural genomics, pyrococcus furiosus, uncharacterized protein, psi, protein structure initiative, southeast collaboratory for structural genomics, secsg, unknown function
• Biological source: Pyrococcus furiosus DSM 3638
• Total number of polymer chains: 1
• Total formula weight: 11819.34

Authors

Liu, Z.J.,Tempel, W.,Chen, L.,Shah, A.,Lee, D.,Clancy-Kelley, L.L.,Dillard, B.D.,Rose, J.P.,Sugar, F.J.,Jenny Jr., F.E.,Lee, H.S.,Izumi, M.,Shah, C.,Poole III, F.L.,Adams, M.W.W.,Richardson, J.S.,Richardson, D.C.,Wang, B.-C.,Southeast Collaboratory for Structural Genomics (SECSG) (deposition date: 2007-04-17, release date: 2007-05-22, Last modification date: 2024-02-21)

Primary citation

Kelley, L.L.,Dillard, B.D.,Tempel, W.,Chen, L.,Shaw, N.,Lee, D.,Newton, M.G.,Sugar, F.J.,Jenney, F.E.,Lee, H.S.,Shah, C.,Poole, F.L.,Adams, M.W.,Richardson, J.S.,Richardson, D.C.,Liu, Z.J.,Wang, B.C.,Rose, J.
Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
Acta Crystallogr.,Sect.F, 63:549-552, 2007

PubMed Abstract: The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

PubMed: 17620707
DOI: 10.1107/S1744309107024049

Experimental method

X-RAY DIFFRACTION (1.85 Å)