Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 47.080, 47.080, 83.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.810 - 1.850 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.24249 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.723 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 9476 | |
| Completeness [%] | 98.5 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.9 | 291 | MODIFIED MICROBATCH USING 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (10 mg/mL) AND A PRECIPITANT SOLUTION CONTAINING 8% PEG 4000 IN 100mM SODIUM ACETATE, pH 3.9, TEMPERATURE 291K, VAPOR DIFFUSION, SITTING DROP |
