Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 47.080, 47.080, 83.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.810 - 1.850 |
R-factor | 0.2083 |
Rwork | 0.207 |
R-free | 0.24249 |
Structure solution method | MIRAS |
RMSD bond length | 0.019 |
RMSD bond angle | 1.723 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 9476 | |
Completeness [%] | 98.5 | 92.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.9 | 291 | MODIFIED MICROBATCH USING 1 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (10 mg/mL) AND A PRECIPITANT SOLUTION CONTAINING 8% PEG 4000 IN 100mM SODIUM ACETATE, pH 3.9, TEMPERATURE 291K, VAPOR DIFFUSION, SITTING DROP |