2PHU
Pterocarpus angolensis lectin in complex with Man-8D1D3
Summary for 2PHU
Entry DOI | 10.2210/pdb2phu/pdb |
Related | 1Q8P 2PHF 2PHR 2PHT 2PHW 2PHX |
Descriptor | Lectin, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose, alpha-D-mannopyranose, ... (6 entities in total) |
Functional Keywords | lectin, carbohydrate, high mannose, sugar binding protein |
Biological source | Pterocarpus angolensis |
Total number of polymer chains | 2 |
Total formula weight | 56495.66 |
Authors | Garcia-Pino, A.,Buts, L.,Wyns, L.,Imberty, A.,Loris, R. (deposition date: 2007-04-12, release date: 2007-07-10, Last modification date: 2024-10-16) |
Primary citation | Garcia-Pino, A.,Buts, L.,Wyns, L.,Imberty, A.,Loris, R. How a plant lectin recognizes high mannose oligosaccharides Plant Physiol., 144:1733-1741, 2007 Cited by PubMed Abstract: The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context. PubMed: 17556509DOI: 10.1104/pp.107.100867 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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