2PHW

Pterocarpus angolensis lectin (PAL) in complex with Man-9

Summary for 2PHW

• Entry DOI: 10.2210/pdb2phw/pdb
• Related: 1Q8P 2PHF 2PHR 2PHT 2PHU 2PHX
• Related PRD ID: PRD_900112
• Descriptor: Lectin, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-alpha-D-mannopyranose, ... (6 entities in total)
• Functional Keywords: lectin, carbohydrate, high mannose, sugar binding protein
• Biological source: Pterocarpus angolensis
• Total number of polymer chains: 2
• Total formula weight: 56801.92

Authors

Garcia-Pino, A.,Buts, L.,Wyns, L.,Imberty, A.,Loris, R. (deposition date: 2007-04-12, release date: 2007-07-10, Last modification date: 2024-10-16)

Primary citation

Garcia-Pino, A.,Buts, L.,Wyns, L.,Imberty, A.,Loris, R.
How a plant lectin recognizes high mannose oligosaccharides
Plant Physiol., 144:1733-1741, 2007

PubMed Abstract: The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.

PubMed: 17556509
DOI: 10.1104/pp.107.100867

Experimental method

X-RAY DIFFRACTION (1.8 Å)