2PBI

The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure

2PBI の概要

• エントリーDOI: 10.2210/pdb2pbi/pdb
• 分子名称: Regulator of G-protein signaling 9, Guanine nucleotide-binding protein subunit beta 5, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: helix wrap, rgs domain, dep domain, dhex domain, ggl domain, beta propeller, signaling protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Membrane : P62881
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 178380.53

構造登録者

Cheever, M.L.,Snyder, J.T.,Gershburg, S.,Siderovski, D.P.,Harden, T.K.,Sondek, J. (登録日: 2007-03-28, 公開日: 2008-01-29, 最終更新日: 2024-02-21)

主引用文献

Cheever, M.L.,Snyder, J.T.,Gershburg, S.,Siderovski, D.P.,Harden, T.K.,Sondek, J.
Crystal structure of the multifunctional Gbeta5-RGS9 complex.
Nat.Struct.Mol.Biol., 15:155-162, 2008

PubMed Abstract: Regulators of G-protein signaling (RGS) proteins enhance the intrinsic GTPase activity of G protein alpha (Galpha) subunits and are vital for proper signaling kinetics downstream of G protein-coupled receptors (GPCRs). R7 subfamily RGS proteins specifically and obligately dimerize with the atypical G protein beta5 (Gbeta5) subunit through an internal G protein gamma (Ggamma)-subunit-like (GGL) domain. Here we present the 1.95-A crystal structure of the Gbeta5-RGS9 complex, which is essential for normal visual and neuronal signal transduction. This structure reveals a canonical RGS domain that is functionally integrated within a molecular complex that is poised for integration of multiple steps during G-protein activation and deactivation.

PubMed: 18204463
DOI: 10.1038/nsmb.1377

実験手法

X-RAY DIFFRACTION (1.95 Å)