2OQN
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Summary for 2OQN
| Entry DOI | 10.2210/pdb2oqn/pdb |
| Descriptor | Thaumatin-1, D(-)-TARTARIC ACID (3 entities in total) |
| Functional Keywords | high pressure cryocooling, capillary cryoprotection, sulfur sad phasing, plant protein |
| Biological source | Thaumatococcus daniellii (miracle fruit) |
| Cellular location | Cytoplasmic vesicle: P02883 |
| Total number of polymer chains | 1 |
| Total formula weight | 22377.15 |
| Authors | Kim, C.U.,Hao, Q.,Gruner, S.M. (deposition date: 2007-01-31, release date: 2007-05-01, Last modification date: 2024-11-13) |
| Primary citation | Kim, C.U.,Hao, Q.,Gruner, S.M. High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths. Acta Crystallogr.,Sect.D, 63:653-659, 2007 Cited by PubMed Abstract: Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography. PubMed: 17452791DOI: 10.1107/S0907444907011924 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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