2OQN
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.7463 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.968, 57.968, 150.716 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.990 - 1.900 |
R-factor | 0.17764 |
Rwork | 0.176 |
R-free | 0.21623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 20909 | |
<I/σ(I)> | 32 | 5 |
Completeness [%] | 99.4 | 93.9 |
Redundancy | 12.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Crystallized in a polycarbonate capillary by vapor diffusion | 7 | 293 | 0.9 M sodium potassium tartrate, pH 7.0, Crystallized in a polycarbonate capillary by vapor diffusion , temperature 293K |