2O7M

The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold

Summary for 2O7M

DescriptorDNA endonuclease I-CreI (2 entities in total)
Functional Keywordshoming endonuclease, dna, hydrolase
Biological sourceChlamydomonas reinhardtii
Cellular locationPlastid, chloroplast P05725
Total number of polymer chains2
Total molecular weight35869.26
Authors
Prieto, J.,Redondo, P.,Padro, D.,Blanco, F.J.,Paques, F.,Montoya, G. (deposition date: 2006-12-11, release date: 2007-10-23, Last modification date: 2011-07-13)
Primary citation
Prieto, J.,Redondo, P.,Padro, D.,Arnould, S.,Epinat, J.C.,Paques, F.,Blanco, F.J.,Montoya, G.
The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage
Nucleic Acids Res., 35:3262-3271, 2007
PubMed: 17452357 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkm183
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliers603.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution