2O7M

The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
A	0006314	biological_process	intron homing
A	0009507	cellular_component	chloroplast
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0004519	molecular_function	endonuclease activity
B	0006314	biological_process	intron homing
B	0009507	cellular_component	chloroplast
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING:

Chain	Residue	Details
A	GLY19
A	ASP20
B	GLY19
B	ASP20

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 233

Chain	Residue	Details
A	GLY19	metal ligand
A	ASP20	metal ligand

---

site_id	MCSA2
Number of Residues	2
Details	M-CSA 233

Chain	Residue	Details
B	GLY19	metal ligand
B	ASP20	metal ligand

---